Application of magnetic field and iron in order to change medicinal products of <Emphasis Type="Italic">Ocimum basilicum</Emphasis>

In the present research, the effects of static magnetic field, with or without iron on the growth, the activity of certain enzymes (like polyphenol oxidase and phenyle alanine ammonialyase), the content of phenolic compounds and the essential oil composition of sweet basil (Ocimum basilicum) were studied. Treatment of plants with static magnetic field with or without iron led to decrease of the growth of plants as well as decrease in the activity of phenyle alanine ammonialyase and of phenolic compounds content. In addition in those plants exposed to magnetic field the amount of essential oils, notably of methyl chavicol, was increased. Exposure of plants to the magnetic field decreased the activity of polyphenol oxidase, whereas treatment of plants with iron when exposed to magnetic field increased polyphenol oxidase activity. In this group the content of essential oil was reduced.     

Characteristics of atmospheric carbonyls and VOCs in Forest Park in South China

The diurnal variation of atmospheric carbonyls and VOCs in a forest in south China were studied in summer 2004. Twenty kinds of carbonyls and eight kinds of VOCs were identified and quantified. Formaldehyde and acetaldehyde were the two most abundant carbonyls, while the most abundant VOCs were isoprene, followed by o-xylene. Most C₃-C₁₀ carbonyls had higher concentrations from 09:00 to 15:00, and their levels were lower during night-time and often reached the lowest in early morning. Formaldehyde and acetaldehyde, however, showed two high levels in their diurnal patterns partly due to their different sources and sinks. The VOCs had different diurnal patterns compared to most carbonyls. The highest concentrations were observed from 03:00 to 06:00 for 1-butene, from 06:00 to 12:00 for isoprene, and from 12:00 to 15:00 for α-pinene. The highest levels for aromatic hydrocarbons occurred during midnight and the lowest in late afternoon. According to the study, emissions from vegetation and photo-oxidation of gas-phase hydrocarbons were the main sources for some carbonyls and VOCs in this region. Other compounds, such as formaldehyde, acetaldehyde and BTEX, showed anthropogenic sources.     

上海化学工业区污水处理厂废气中挥发性有机物主要成分的测定

建立了使用活性炭管采集臭气中的挥发性有机物,经二硫化碳解吸,用GC-MS仪Scan扫描方式确定挥发性有机物的组分后优化GC-MS条件定性定量分析上海化学工业区污水处理厂臭气中主要挥发性有机物的方法。结果表明,从臭气中检测出11种VOCs,线性相关系数R2均大于0.99,相对标准偏差为3.0%～4.7%,除了苯乙烯、α-甲基苯乙烯和4-甲基苯乙烯的解吸效率依次分别为72%、74%和66%,其他挥发性有机物的解吸效率均为91%～100%。本方法操作简便,能够有效分离和准确测定臭气中挥发性有机物,具有较低的检出限和较好的精确度,适合臭气中挥发性有机物的检测分析,可为臭气控制提供可靠的数据。     

NUMERICAL MODEL OF A TRACER TEST ON THE SANTA CLARA RWER,VENTURA COUNTY,CALIFORNIA1

ABSTRACT: To better understand the flow processes, solute-trans. port processes, and ground-water/surface-water interactions on the Santa Clara River in Ventura County, California, a 24-hour fluorescent-dye tracer study was performed under steady-state flow conditions on a 45-km reach of the river. The study reach includes perennial (uppermost and lowermost) subreaches and ephemeral subreaches of the lower Piru Creek and the middle Santa Clara River. The tracer-test data were used to calibrate a one-dimensional flow model (DAFLOW) and a solute-transport model (BLTM). The dye-arrival times at each sample location were simulated by calibrating the velocity parameters in DAFLOW. The simulations of dye transport indicated that (1) ground-water recharge explains the loss of mass in the ephemeral middle subreaches, and (2) ground-water recharge does not explain the loss of mass in the perennial uppermost and lowermost subreaches. The observed tracer curves in the perennial subreaches were indicative of sorptive dye losses, transient storage, and (or) photodecay - these phenomena were simulated using a linear decay term. However, analysis of the linear decay terms indicated that photodecay was not a dominant source of dye loss.     

Integral assessment of estrogenic potentials in sediment-associated samples

Background, aim, and scope  The enzyme-linked receptor assay (ELRA) detects estrogenic and anti-estrogenic effects at the molecular level of receptor binding and is a useful tool for the integrative assessment of ecotoxicological potentials caused by hormonally active agents (HAA) and endocrine disrupting compounds (EDC). The main advantage of the ELRA is its high sample throughput and its robustness against cytotoxicity and microbial contamination. After a methodological adaptation to salinity of the ELRA, according to the first part of this study, which increased its salinity tolerance and sensitivity for 17-β-estradiol, the optimised ELRA was used to investigate 13 native sediments characterised by different levels of salinity and chemical contamination. The applicability of the ELRA for routine analysis in environmental assessment was evaluated. Salinity is often a critical factor for bioassays in ecotoxicological sediment assessment. Therefore, salinity of the samples was additionally adjusted to different levels to characterise its influence on elution and binding processes of receptor-binding substances. Materials and methods  The ELRA was carried out with the human estrogen receptor α (ER) in a 96-well microplate format using the experimental setup known from the competitive immunoassay based on ligand–protein interaction. It is an important improvement that a physiologically relevant receptor was used as a linking protein instead of an antibody. The microplates were coated with a 17-β-estradiol-BSA conjugate, and dilution series of estradiol and of native sediment samples were added and incubated with the ER. After a washing step, a biotinylated mouse anti-ER antibody was added to each well. Receptor binding to estradiol, agonistic and antagonistic receptor binding, were determined by a streptavidin-POD-biotin complex with subsequent measurement of the peroxidase activity at the wavelength of 450 nm using a commercial ELISA multiplate reader. The sediment elutriates and pore water samples of sediments were tested in a dilution series to evaluate at which dilution step the receptor-binding potential ends. In the elution process (see Section 2.1 to 2.2), a method was developed to adjust the salinity to the levels of the reference testings, which offers an appropriate option to adjust the salinity in both directions. Statistical evaluation was made with a combination of the Mann–Whitney U test and the pT-method. Results  This part of the study characterised the environmental factor ‘salinity’ for prospective applications of the ELRA. Using reference substances such as 17-β-estradiol, the ELRA showed sigmoid concentration-effect relations over a broad range from 0.05 μg/l to 100 μg/l under physiological conditions. After methodological optimisation, both sensitivity and tolerance of the assay against salinity could be significantly raised, and the ELRA became applicable under salinity conditions up to concentrations of 20.5‰. The mean relative inter-test error (n = 3) was around 11% with reference substances and below 5% for single sediments elutriates in three replicates each. For sediment testings, the pore water and different salinity-adjusted elutriates of 13 sediments were used. A clear differentiation of the receptor-binding potential could be reached by application of the pT-method. Thereby, pT-values from one to six could be assigned to the sediments, and the deviation caused by the different salinity conditions was one pT-value. The mean standard deviation in the salinity adaptation procedure of the elutriates was below 5%. Discussion  Although the ELRA has already been used for assessments of wastewater, sludge and soil, its applicability for samples to different salinity levels has not been investigated so far. Even if the ELRA is not as sensitive as the E-screen or the YES-assay, with regard to reference substances like 17-β-estradiol, it is a very useful tool for pre-screening, because it is able to integrate both estrogenic as well as anti-estrogenic receptor-binding effects. According to the results of sediment testing, and given the integrative power to detect different directions of effects, the ELRA shows sufficient sensitivity and salinity tolerance to discriminate receptor-binding potentials in environmental samples. Conclusions  The optimised ELRA assay is a fast, cost-effective, reliable and highly reproducible tool that can be used for high-throughput screening in a microplate format in detecting both estrogenic and anti-estrogenic effects. Additionally, the ELRA is robust against microbial contaminations, and is not susceptible towards cytotoxic interferences like the common cell-culture methods. The general applicability and sufficient sensitivity of the ELRA was shown in freshwater environments. Marine and brackish samples can be measured up to salinity levels of 20.5‰. Recommendations and perspectives  In view of the proven sensitivity, functionality and the fastness of the ELRA, it is recommendable to standardise the test method. At the moment, no adequate in vitro test procedure exists which is standardised to DIN or ISO levels. The E-screen and the yeast estrogen/androgen screens (YES/YAS) sometimes underlie strong cytotoxic effects, as reported in the first part of this study. Further development of an ELRA assay using human androgen receptors appears to be very promising to gain information about androgenic and anti-androgenic effects, too. This would offer a possibility to use the ELRA as a fast and reliable pre-screening tool for the detection of endocrine potentials, thus minimising time and cost-expensive animal experiments.     

联苯代谢对微生物的生长胁迫及分裂抑制

以联苯/多氯联苯降解菌株R04（Rhodococcus sp.R04）和几种模式微生物为研究对象，利用高效液相色谱、荧光显微镜、扫描电子显微镜等分析微生物在联苯及其代谢物培养条件下细胞分裂和形态的变化.结果表明联苯及其代谢产物对红球菌R04和几种模式微生物细胞的分裂有抑制作用，并对部分微生物形态有影响.与前体-联苯及其代谢产物2-羟基-6-酮基-6-苯基-2，4-己二烯酸相比，2，3-二羟基联苯对G⁺、G^-细菌，或是酵母细胞分裂都有较强的抑制和形态的改变.2，3-二羟基联苯导致R.R04和缺陷型R.R04细胞形成不完整隔膜的比例增加；造成96.4%的大肠杆菌BL21细胞表面凹陷，胞质内容物流失，菌体体积缩小；导致枯草芽孢杆菌89.6%的细胞体积明显缩小；导致金黄色葡萄球菌基本没有细胞能形成完整的分裂隔膜；导致红酵母细胞能进行出芽生殖的比例从64.2%降低到19.3%，但对其细胞形态无明显改变.联苯代谢物2，3-二羟基联苯对红球菌R04及其它微生物细胞分裂和增殖的抑制作用比其前体-联苯强，建议在研究环境化合物与微生物互作时，应考虑环境化合物代谢的毒性效应.     

室内空气有机污染的研究现状

主要讨论了室内空气中有机物污染的研究现状。重点介绍了室内空气中多环芳烃 (PAHs)、挥发性有机物(VOCs)、醛类化合物等的污染状况及来源。简要叙述了室内空气污染的影响因素及对人体的健康风险评价。     

含氯代烃废水的生物毒性与处理方法探讨

卤代烃是在有机物分子中的碳原子上，用卤素基团取代出氢的卤化产物，这个变化使有机物的生物毒性增大，这是卤素有机态毒性的体现；另一方面，卤代烃在生物水解或降解过程中，又会重新释放出带正电荷的卤素，与水结合后成为次卤酸而具有无机态卤素的生物毒性。作者在提出这种卤代烃生物毒性学说的基础上，提出了一系列在含卤代烃废水预处理与生物处理中的解毒、降毒、抗毒和减荷及提高可生化性的措施，以提高含卤代烃废水的综合处理效率。     

季铵化合物对好氧活性污泥吸附Ni2+的影响研究

以季铵化合物中最具潜在影响力、毒性较大的苄基季铵盐(BAC)为模型物,进行了BAC对好氧活性污泥吸附Ni2+的影响研究.结果表明,当反应液初始pH≤3时,不同浓度的BAC对好氧活性污泥吸附Ni2+的影响较小,当初始pH在3～9时,不同浓度的BAC对Ni2+吸附的抑制影响顺序为100 mg/L＞20 mg/L＞0 mg/...     

Seaweed sex pheromones and their degradation products frequently suppress amphipod feeding but rarely suppress sea urchin feeding

Summary. A diverse group of brown seaweeds produce bouquets of C₁₁ metabolites, some of which act as pheromones that cue gamete release or attract sperm to eggs following release. We demonstrate that these C₁₁ metabolites and their degradation products also frequently and strongly deter feeding by the herbivorous amphipod Ampithoe longimana, but rarely by the herbivorous sea urchin Arbacia punctulata. Across the range of concentrations tested, seven of twelve C₁₁ metabolites or mixtures that we tested deterred feeding by the amphipod, but only two of eleven deterred the sea urchin. For those compounds where we could rigorously contrast the magnitude of deterrence against the amphipod with the magnitude of deterrence against the urchin, the amphipod was deterred significantly more than the urchin by five of six metabolites. Thus, C₁₁ compounds were more frequently and more strongly deterrent to the amphipod than to the sea urchin. These findings for C₁₁ metabolites conflict with previous investigations, where other classes of seaweed chemical defenses have been shown to deter feeding by large mobile herbivores like urchins and fishes but to be relatively ineffective against mesograzers, especially the species of amphipod that we used here. Our results suggest that C₁₁ metabolites are unusual among the known seaweed chemical defenses in that they are especially effective against mesograzers, which often consume seaweed spores, zygotes, and juveniles. The high concentrations of C₁₁ metabolites in brown algal eggs could allow these defenses to be especially important in defending gametes, zygotes, or young sporelings from herbivorous mesograzers. Received 26 February 1998; accepted 9 April 1998.